Polydnaviruses, double-stranded DNA viruses with segmented genomes, have evolved as obligate endosymbionts of parasitoid, and plays a very important role in the life cycle of parasitoids. The PDV symbiotic with different endoparasites are often different. Here we completed the the six protein tyrosine phosphatases (PTPs) and six ankyrins sequence of a PDV which from Microplitis similis, by sequencing and compared the gene features of this PDV to those of other polydnaviruses. Phylogenetic analysis indicated that it was closely related to Microplitis demolitor BV (MdBV) and Microplitis mediator BV (MmBV), and this result was supported by nucleotide multiple sequence alignment. So a new species of PDV-MsBV was identified and characterized in Microplitis similis. It provides a new material for the co-evolution of biology. At the same time, the structure and function of these 12 proteins were predicted and analyzed. The results showed a new perspective on the study of PTPs and ankyrins protein family in PDVs, and provide the materials and foundations for analysizing the function of MsBV.

Residues of bovine serum albumin in viral vaccines can cause allergic reactions. The quality control of bovine serum is a prerequisite for ensuring the safety and reliability of the vaccine. Domestic and foreign regulatory authorities have made clear limits on bovine serum residues.