Research and Implementation of Real Time Fluorescent Nucleic Acid Amplification Detection Technology

Abstract

This paper introduces the research results of real-time detection of fluorescent nucleic acid amplification technology, mainly including the cooling rate, the precision of temperature control, temperature accuracy, module temperature uniformity, fluorescence intensity detection repeatability and measurement precision and the threshold cycle number. The working principle is based on the operating parameters of computer input, controlling module component temperature placed reagent sample changes, meeting the reagent sample high temperature denaturation, annealing temperature (complex), suitable temperature extension process cycle the reagent sample amplification, and through the photoelectric detection system according to the set of sample fluorescence changes. Analysis of data is processed by software. The results can be used for measurement of polymerase chain reaction and fluorescence quantitative examination of clinical diagnosis fields of immunology, the human genome engineering, forensic medicine, oncology, organization and population biology, paleontology, botany, animal science research field and virus, tumor, genetic diseases.