Immunogenicity Evaluation of Virus-like Particles of Enterovirus 71 in Hansenula polymorpha

Abstract

Objective The virus-like particles（VLPs）of enterovirus 71（EV71）expressed in Hansenula polymorpha, EV71 vaccine was prepared by different test design and the immunogenicity was evaluated. Methods Vaccine stock solution with recombinant was obtained from fermented product processed through series of purification operations. EV71 vaccine was prepared with VLPs and the serum neutralizing antibodies（NTAbs）was determinated to evaluate the immunogenicity of different lines of mice, different aluminum adjuvants and different immune doses. Results EV71 vaccine was prepared with aluminum hydroxide and immunized SPF NIH mice were more sensitive to determinate NTAbs. Different immune doses of EV71 vaccine all have good immunogenicity, and the second immunization can enhance the immune effect significantly, and the NTAbs increases gradually with the increase of immune dose. The results showed that the immunogenicity in mice was dose-effect relationship with the immune dose. Conclusion EV71 VLPs vaccines prepared in this study have good immunogenicity, which provide reference data for the subsequent formulation and the development of polyvalent vaccine.