Development and Evaluation of Colloidal Gold Immunochromatographic Strip for Rapid Detection of Canine Parainfluenza Virus Type 5 Strain

Jingyun Wang, Liying Hao, Fan Li, Chenyu Bai, Jingjing Bai, Dingding Zheng, Yujiao Cao, Xiangdong Li, Yuxiu Liu, Junhua Deng, Kegong Tian

Abstract


A colloidal gold immunochromatographic test strip was firstly developed to detect Canine Parainfluenza Virus type 5 based on a sandwich format. The sensitivity of strip was tested for 103.8 TCID50 mL-1 to CPIV 5. No cross-reactivity with five common viruses from dogs were observed. Thirty-nine nasopharyngeal swabs samples were used to compare the consistency between RT-PCR and the strip, and the total coincidence rate was 87.18%. The colloidal gold immunochromatographic test strip thus provides a rapid and convenient method for on-site detection of CPIV 5 without any ancillary equipment.


Keywords


canine parainfluenza virus type 5; monoclonal antibody; colloidal gold; strip

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DOI: https://doi.org/10.21092/jav.v8i2.109

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