The development of ELISA Kit detecting Chikungunya Virus used a synthetic polypeptide chain

Abstract

Chikungunya fever was an acute vector-infectious disease caused by Chikungunya virus which outbreaks were distributed mostly in Africa, Indian Ocean, West Pacific Islands and South-East Asia. It was important to develop a rapid and accurate detection kit to control the Chikungunya virus transmission. The ELISA method was a valuable tool for rapid diagnosis of acute viral infections which had the merits of rapid, accurate, simple. In present study, a polypeptide chain of E2 protein was chosen and validated through computer protein model analysis, and the Chikungunya virus ELISA kit detecting the E2 antigen was developed and evaluated based this polypeptide. The Sandwich method Chikungunya antigen ELISA kit consisted of polyclonal antibody made by Chikungunya virus synthetic protein and a commercial monoclonal antibody. The sensitivity of antigen detection was about 100ng/mL of pure synthetic protein. The specificity and reproducibility of the ELISA kit were also validated and the results were as followed. This detection method not merely gave us a positive confirmatory result in early phase of the disease, but also practical in the diagnosis of prodromal and subclinical stage and might be useful for the rapid detection of Chikungunya virus from vector.