Neurotoxicity of Japanese encephalitis Virus chimeric with dengue type 2 virus strain on sucking mice

Abstract

The aim is to indentify the safety of JE/DV2 chimeric virus by neurovirulence experiment with sucking mice.  Chimeric viruses, DV2 and JEV were diluted. TCID50 of diluted virus liquid were from 10^-5to 10^-1. Sucking mice were injected intracerebrally with every type of viruses at each dose level and then were observed for 14 days. Survival curve were compared between different groups by Log-rank test. The results showed that survival rate of the sucking mice in two chimeric virus groups were lower than those of JEV group (p<0.05). Compared with DV2 group, survival rate of the sucking mice in two chimeric virus groups were lower than those of DV2 group (p<0.05) in some levels of dose. However, in some levels of dose there were no statistical significance between the chimeric virus group and DV2 group. Results of sucking mice experiment about neurotoxicity indicated that the rescued chimeric virus was not attenuated.